Areas of Scientific Interest
Host-pathogen interactions, CRISPR, genome editing, CRISPR screening, bacterial toxins, functional genomics/transcriptomics, C. elegans, metagenomics.
Main Activities / Research Directions
- Host-pathogen interactions, focusing on pathogenic bacteria and their toxins
- Development of CRISPR screening technologies
- C. elegans and wild nematode interactions with bacteria
- C. elegans genetics
- Bacterial metagenomics
Research
- CRISPR screening
- Genome-wide CRISPR knock-out screening
Genome-wide CRISPR activation screening
Transcriptome-wide CRISPR knock-down screening - Bacterial toxins
Cholesterol-dependent cytolysins
TlyC hemolysins
Diphtheria and Cholera toxins - Bacterial metagenomics
CRISPR-Cas system ecology
Novel CRISPR-Cas system discovery
Toxin discovery
Enzyme discovery
Methods and Infrastructure Used
- Cell culture + Viral prep/transductions
- CRISPR/RNAi screening
- Genomics and transcriptomics (WGS, RNA-seq, sRNA-seq)
- Fluorescence-activated cell sorting (FACS)
- Next generation sequencing (Illumina, Element, Ultima)
- Fluorescence microscopy
- Bioinformatics/High-performance computing (RNA-seq, genome assembly, variant calling, eQTL analysis, protein discovery, metagenome analysis)
Cooperation
KU Leuven, Rega Institute, Belgium
The University of Glasgow, Scottland, UK
The Rockefeller University, USA
List of Employees and Contacts
|
Academic degree |
Name |
Contacts |
|
Dr. |
Gediminas Drabavičius (Head of Laboratory) |
|
| Aušrinė Junčytė |
Information for Those Interested in Cooperation
Cooperation and Services: host-pathogen laboratory studies how bacteria and other pathogens interact with the host cells (Human, nematode, bacteria) utilizing gene editing and CRISPR screening technologies. Lab provides services and is seeking cooperation in: gene editing in mammalian cells, Genome-wide CRISPR screening (CRISPR KO, activation), new generation sequencing and analysis, genome editing and CRISPR screening consultations.
Keywords: CRISPR; genome-wide; screening; Cas9; Cas12; Cas13; New Generation Sequencing; NGS, toxins; C. elegans; lentivirus; transduction.
For cooperation and partnerships at the Translational Health Research Institute, please contact: